The microscopic observation of lung tissue is challenging due to its fragile nature.Xylene and isopropanol are common tissue-clearing reagents used before paraffin embedding, yet no studies have compared these two reagents in lung tissue processing.Due to the well-known health risks xylene could introduce to operators, as well as its environmental
Abstract Cryo-focused ion beam (cryo-FIB) milling technology has been developed for the fabrication of cryo-lamella of frozen native specimens for study by in situ cryo-electron tomography (cryo-ET).However, the precision of the target of interest is still one of the major bottlenecks limiting application.Here, we have developed a cryo-correlative
Chocolate is a product of the fermentation Toners of cacao beans.Performed on-farm or at local cooperatives, these are spontaneous cacao fermentations (SCFs).To better understand SCFs, this study sought to identify SCF microbes, their interrelationships, and other key parameters that influence fermentation.This is important because differences in f
Overlap syndrome, which is known as the coexistence of chronic obstructive pulmonary disease (COPD) and obstructive sleep apnea (OSA), was first defined by Flenley.Although it can refer to concomitant occurrence of any of the pulmonary diseases and OSA, overlap syndrome is commonly considered as the coexistence of OSA and COPD.This disease has uniq